Nucleic acid amplification is one of the most commonly performed molecular
biology techniques and is a necessary precursor to a range of methods from
gene cloning and site-directed mutagenesis to the quantitative analysis of
gene expression. The Nucleic Acid Amplification Dashboard was developed from
responses to a 21-question survey by 485 scientists predominantly located in
North America and Europe. This Dashboard reveals key market indicators for the
nucleic acid amplification market as a whole as well as for the following
sub-segments: Standard PCR with Taq polymerase, hot start Taq polymerase and
proofreading polymerases
qPCR with Taq polymerase and with hot start Taq polymerase
qRT-PCR with Taq polymerase and with hot start Taq polymerase
Instruments for Standard and Real Time PCR
Survey Methodology
ln June of 2009, Percepta fielded the Nucleic Acid Purification Survey to a
subset of the Percepta BioAnalytix™ Panel of life scientists.
Individuals were invited by e-mail blast to click-through to a webpage at
perceptabioanalytix.com where the survey was hosted. Invitations were
delivered beginning onJune 1,2009 and results collected throughJune 11, 2009.
A total of 485 scientists participated in the survey, of which 442 are
actively engaged in performing nucleic acid amplification and 12 plan to use
nucleic acid amplification methodsin the next 12 months. Results based on the
aggregate of collected responses are revealed in this Nucleic Acid
Amplification Dashboard.
Table 19: Respondent' s Primary Supplier for qRT-PCR (cDNA template) using
Taq Polymerase by Market Segment
Table 20: Respondent' s Primary Supplier for qRT-PCR (cDNA template) using
a Hot Start Taq Polymerase by Market Segment
Table 21: Market Share Leaders for RT-PCR (cDNA) Amplification Products
Table 22: Respondent' s Primary Supplier for Standard PCR Thermocyclers by
Market Segment
Table 23: Respondent' s Primary Supplier for Instruments for Standard
Real-Time PCR by Market Segment
Table 24: Market Share Leaders for Standard & RT-PCR Instruments
Table 25: Percentage of Respondents Satisfied with Various Nucleic Acid
Amplification Product Categories and Reasons for Interest in Switching
Table 26: Percentage of Respondents Satisfied with Nucleic Acid
Amplification Product Categories - Comparison to 2007 Nucleic Acid
Amplification Dashboard
Table 27: Most Important Features of Products for Nucleic Acid
Amplification - Comparison to 2007 Nucleic Acid Amplification Dashboard
Table 28: Respondent' s Primary & Secondary Applications for End Products
from Standard PCR with Taq Polymerase - Comparison to 2007 Nucleic Acid
Amplification Dashboard
Table 29: Respondent' s Primary & Secondary Applications for End Products
from Standard PCR with a Proofreading (High Fidelity) Polymerase - Comparison
to 2007 Nucleic Acid Amplification Dashboard
Table 30: Respondent' s Primary & Secondary Applications for End Products
from qPCR (genomic DNA template) using Taq Polymerase - Comparison to 2007
Nucleic Acid Amplification Dashboard
Table 31: Respondent' s Primary & Secondary Applications for End Products
from qRT-PCR (cDNA template) using Taq Polymerase - Comparison to 2007 Nucleic
Acid Amplification Dashboard