Abstract
Description
Overview
Nucleic acid amplification is one of the most commonly performed molecular
biology techniques and is a necessary precursor to a range of methods from
gene cloning and site-directed mutagenesis to the quantitative analysis of
gene expression. The Nucleic Acid Amplification Dashboard was developed from
responses to a 21-question survey by 492 scientists predominantly located in
North America and Europe. This Dashboard reveals key market indicators for
the nucleic acid amplification market as a whole as well as for the following
sub-segments:
- Difficult PCR (e.g. GC rich, complex, long targets)
- Digital PCR
- Emulsion PCR
- Fast PCR
- High Fidelity PCR
- High Resolution Melt (using real time PCR instrument)
- Standard endpoint PCR
- Standard endpoint RT-PCR
- qPCR (real time PCR with genomic DNA template)
- qRT-PCR (real time PCR with RNA or cDNA template)
While standard protocols may be well-defined, amplification markets are quite
fluid, with significant influence from such external sources as:
- An evolving intellectual property landscape
- New pricing strategies from established suppliers
- Application to emerging research areas such as gene silencing, SNP
genotyping and rapid, whole genome sequencing
In order to dive more deeply into the characteristics and dynamics of the
market for nucleic acid amplification products, Percepta has introduced the
Nucleic Acid Amplification Dashboard, designed to take a snapshot of the
current market landscape with the future goal of repeating and publishing the
study to give Dashboard readers the ongoing story of how the market is
adapting to new products, new competitors and new sales and marketing
strategies.
Survey Methodology
In July of 2011, Percepta fielded the Nucleic Acid Amplification Survey to a
subset of the Percepta BioAnalytix"! Panel of life scientists. Individuals
were invited by e-mail blast to click-through to a webpage at
perceptabioanalytix.com where the survey was hosted. Invitations were
delivered beginning on July 22, 2011 and results collected through August 3,
2011. A total of 492 scientists participated in the survey, of which 477 are
actively engaged in performing nucleic acid amplification and 6 plan to use
nucleic acid amplification methods in the next 12 months. Results based on
the aggregate of collected responses are revealed in this Nucleic Acid
Amplification Dashboard.
Respondent Demographics
Respondents from the academic, government and commercial market segments are
well represented, with 18.9% of respondents employed in an industry setting.
71.7% of respondents are from North America, while 26.7% reside in Europe.
Junior (Lab Technician, Graduate Student), mid-level (Post-Doctoral Fellow,
Lab Manager) and senior (Professor/PI, Group Leader) scientists are well
represented in the data set, with the most cited job titles being
Professor/PI/Instructor (21.3% of respondents), Scientist/Senior Scientist
(18.9% of respondents) and Post-Doctoral Fellow/Research Fellow (17.3% of
respondents).
A wide variety of scientific areas of specialization is also evident, led by
cell biology (named by 18.3% of respondents as their primary area of
expertise), followed by biochemistry (15.9%) and microbiology/infectious
disease/virology (12.0%).
Small (1-5 scientists), medium (6-20 scientists) and large (>20 scientists)
laboratories are well represented: 55.3% of respondents work in labs where 1
to 5 people perform nucleic acid amplification experiments at least a few
times each year; 37.6% in labs with 6 to 20 experimenters, and the remaining
7.1% in labs with greater than 20 bench scientists.
Table of Contents
- Executive Summary
- Key Findings and Implications
- Nucleic Acid Amplification Dashboard
- Nucleic Acid Amplification Market Opportunity Matrix
- Survey Methodology
- Survey Invitation Text
- Respondent Demographics
- Frequency of Performance of Life Science Techniques
- Frequency of Performance of Nucleic Acid Amplification
- Reaction Throughput and Market Growth Rates
- Respondents' Stated Price per Reaction
- Total Market Size, Market Segment Sizes and Total Market Growth Rate
- Market Shares by Segment (Share of Mentions)
- Customer Satisfaction and Interest in Switching Suppliers
- Product Features That Influence Purchasing Decisions
- Primary and Secondary Downstream Applications
- Desired Changes to Nucleic Acid Amplification Products
- Survey Questionnaire
- Appendix
Figures and Tables
- Figure 1: Respondents' Place of Employment
- Figure 2: Respondent' s Location
- Figure 3: Respondents' Job Title
- Figure 4: Respondents' Areas of Expertise/Specialization
- Figure 5: Number of Employees in Respondents' Laboratories
- Figure 6: Percentage of Respondents Performing Various Techniques at Least
a Few Times per Year
- Figure 7: Percentage of Respondents Performing Nucleic Acid Amplification
- Figure 7A: Change in Percentage of Respondents Performing Nucleic Acid
Amplification
- Figure 8: Percentage of Respondents Performing Various Nucleic Acid
Amplification Techniques at least a Few Times per Year
- Figure 9: Percentage of Respondents Using Various Thermostable Polymerases
for Nucleic Acid Amplification Techniques
- Figure 10: Percentage of Respondents Performing Difficult PCR
- Figure 11: Percentage of Respondents Performing Digital PCR
- Figure 12: Percentage of Respondents Performing Emulsion PCR
- Figure 13: Percentage of Respondents Performing Fast PCR
- Figure 14: Percentage of Respondents Performing High Fidelity PCR
- Figure 14A: Change in Percentage of Respondents Performing High Fidelity
PCR
- Figure 15: Percentage of Respondents Performing High Resolution Melt
- Figure 16: Percentage of Respondents Performing Standard Endpoint PCR
- Figure 17: Percentage of Respondents Performing Standard Endpoint RT-PCR
- Figure 18: Percentage of Respondents Performing qPCR
- Figure 19: Percentage of Respondents Performing qRT-PCR
- Figure 20: Respondents' Primary Supplier for Difficult PCR
- Figure 21: Respondents' Primary Supplier for Digital PCR
- Figure 22: Respondents' Primary Supplier for Emulsion PCR
- Figure 23: Respondents' Primary Supplier for Fast PCR
- Figure 24: Respondents' Primary Supplier for High Fidelity PCR
- Figure 24A: Change in Mention for Respondents' Primary Supplier for High
Fidelity PCR
- Figure 25: Respondents' Primary Supplier for High Resolution Melt
- Figure 26: Respondents' Primary Supplier for Standard Endpoint PCR
- Figure 27: Respondents' Primary Supplier for Standard Endpoint RT-PCR
- Figure 28: Respondents' Primary Supplier for qPCR
- Figure 29: Respondents' Primary Supplier for qRT-PCR
- Figure 30: Respondents' Primary Supplier of Instruments for Standard PCR
- Figure 30A: Change in Respondents' Primary Supplier of Instruments for
Standard PCR
- Figure 31: Respondents' Primary Supplier of Instruments for Real Time PCR
- Figure 31A: Change in Respondents' Primary Supplier of Instruments for
Real Time PCR
- Figure 32: Respondents' Primary and Secondary Applications for End
Products from Difficult PCR
- Figure 33: Respondents' Primary and Secondary Applications for End
Products from Digital PCR
- Figure 34: Respondents' Primary and Secondary Applications for End
Products from Emulsion PCR
- Figure 35: Respondents' Primary and Secondary Applications for End
Products from Fast PCR
- Figure 36: Respondents' Primary and Secondary Applications for End
Products from High Fidelity PCR
- Figure 37: Respondents' Primary and Secondary Applications for End
Products from High Resolution Melt
- Figure 38: Respondents' Primary and Secondary Applications for End
Products from Standard Endpoint PCR
- Figure 39: Respondents' Primary and Secondary Applications for End
Products from Standard Endpoint RT-PCR
- Figure 40: Respondents' Primary and Secondary Applications for End
Products from qPCR
- Figure 41: Respondents' Primary and Secondary Applications for End
Products from qRT-PCR
- Figure 42: Non-current users of Nucleic Acid Amplification Products Future
Plans
List of Tables
- Table 1: Respondents' Area of Expertise (Values for Figure 4)
- Table 2: Frequency of Performance of Various Life Science Techniques
- Table 3: Frequency of Co-Performance of Various Life Science Techniques
- Table 4: Percentage of Respondents Performing Nucleic Acid Amplification
by Place of Employment and Location
- Table 5: Frequency of Performance of Various Nucleic Acid Amplification
Techniques
- Table 6: Frequency of Co-Performance of Life Science Techniques with
Nucleic Acid Amplification Techniques
- Table 7: Frequency of Co-Performance of Nucleic Acid Amplification
Techniques with Life Science Techniques
- Table 8: Mean, Median and Trim Mean Monthly Throughput for Nucleic Acid
Amplification Techniques
- Table 8A: Change in Median Monthly Throughput for Nucleic Acid
Amplification Methods - Comparison to the 2009 Dashboard
- Table 8B: Mean, Median and Trim Mean Monthly Throughput for Nucleic Acid
Amplification Methods by Organization Type
- Table 9: Percentage of Respondents Performing Probe-based vs. DNA
Binding-dye based Reactions for real time PCR
- Table 10: Percentage of Respondents Performing Various Numbers of
Amplification Reactions Per Month by Product Category
- Table 11: Projected Growth in the Performance of Various Nucleic Acid
Amplification Techniques
- Table 12: Mean, Median and Trim Mean Price per Reaction for Nucleic Acid
Amplification Techniques
- Table 13: Change in Mean, Median and Trim Mean Price per Reaction for
Nucleic Acid Amplification Techniques
- Table 14: Estimated 2012 Global Market Size for Nucleic Acid Amplification
Techniques
- Table 15: Respondents' Primary Supplier for Difficult PCR by Market Segment
- Table 16: Respondents' Primary Supplier for Digital PCR by Market Segment
- Table 17: Respondents' Primary Supplier for Emulsion PCR by Market Segment
- Table 18: Respondents' Primary Supplier for Fast PCR by Market Segment
- Table 19: Respondents' Primary Supplier for High Fidelity PCR by Market
Segment
- Table 20: Respondents' Primary Supplier for High Resolution Melt by Market
Segment
- Table 21: Respondents' Primary Supplier for Standard Endpoint PCR by
Market Segment
- Table 22: Respondents' Primary Supplier for Standard Endpoint RT-PCR by
Market Segment
- Table 23: Respondents' Primary Supplier for qPCR by Market Segment
- Table 24: Respondents' Primary Supplier for qRT-PCR by Market Segment
- Table 25: Market Share Leaders for Nucleic Acid Amplification Products
- Table 26: Respondents' Primary Supplier of Standard PCR Thermocyclers by
Market Segment
- Table 27: Respondents' Primary Supplier for Instruments for Real Time PCR
by Market Segment
- Table 28: Market Share Leaders for Standard and Real Time PCR Instruments
- Table 29: Percentage of Respondents Satisfied with Various Nucleic Acid
Amplification Techniques and Reasons for Dissatisfaction
- Table 30: Percentage of Respondents That Have Switched Suppliers in the
Last Six Months
- Table 31: Most Important Features of Nucleic Acid Amplification Products
- Table 31A: Most Important Features of Nucleic Acid Amplification Products
- Comparison to 2009 Nucleic Acid Amplification Dashboard
- Table 32: Respondents' Primary and Secondary Applications for End Products
from Difficult PCR (Values for Figure 32)
- Table 33: Respondents' Primary and Secondary Applications for End
Products from Digital PCR (Values for Figure 33)
- Table 34: Respondents' Primary and Secondary Applications for End
Products from Emulsion PCR (Values for Figure 34)
- Table 35: Respondents' Primary and Secondary Applications for End
Products from Fast PCR (Values for Figure 35)
- Table 36: Respondents' Primary and Secondary Applications for End Products
from High Fidelity PCR (Values for Figure 36)
- Table 37: Respondents' Primary and Secondary Applications for End
Products from High Resolution Melt (Values for Figure 37)
- Table 38: Respondents' Primary and Secondary Applications for End
Products from Standard Endpoint PCR (Values for Figure 38)
- Table 39: Respondents' Primary and Secondary Applications for End Products
from Standard Endpoint RT-PCR (Values for Figure 39)
- Table 40: Respondents' Primary and Secondary Applications for End Products
from qPCR (Values for Figure 40)
- Table 41: Respondents' Primary and Secondary Applications for End Products
from qRT-PCR (Values for Figure 41)
- Table 42: Respondents' Primary Applications for End Products from Nucleic
Acid Amplification
