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市場調查報告書

單一細胞技術趨勢

Single Cell Technologies Trends 2014

出版商 HTStec Ltd 商品編碼 246168
出版日期 內容資訊 英文 60 Pages
商品交期: 最快1-2個工作天內
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單一細胞技術趨勢 Single Cell Technologies Trends 2014
出版日期: 2014年10月01日 內容資訊: 英文 60 Pages
簡介

本報告由各種產業利用或計劃利用單一細胞及單一細胞技術的人、團體為對象所進行的調查結果,為您彙整目前單一細胞相關技術的使用概況及利用計劃(利用領域·用途等),利用的偏好,阻礙利用的限制,未滿足需求,改良的要求,設備及消耗品的預算等資料。

  • 摘要整理
  • 目錄
  • 調查方法
  • 受訪者所屬企業·調查的回答
  • 受訪者的出身地區
  • 受訪者的所屬企業/組織
  • 受訪者的負責業務
  • 現在利用單一細胞及單一細胞技術的受訪者
  • 單一細胞技術的關心與利用領域
  • 受訪者的單一細胞研究用途類別化
  • 最適合關注單一細胞技術的研究領域
  • 單一細胞技術的利用或用途之重要性
  • 利用單一細胞或平均化母集團的主要理由
  • 從單一細胞技術所抽出的特定資訊的價值
  • 從單一細胞技術所抽出的資訊:是否可與替代方法比較?
  • 組織或液體培地的單一細胞分離上,目前所利用的技術
  • 單一細胞適用的分析技術
  • 符合目的的單一細胞解析技術
  • 調查結果的摘要
  • 單一細胞技術用途所使用的細胞類型
  • 單一細胞的分離所使用的來源
  • 單一分離細胞下所容許的純度水準
  • 下游的分離細胞相關工作
  • 單一細胞的用途
  • 一般認為影響單一細胞技術的用途
  • 重要性巨大的單一細胞技術的功能
  • 單一細胞的目前潛在性用途
  • 現在可以利用的單一細胞分離技術之主要限制
  • 調查結果的摘要
  • 單一細胞設備的購買可能性
  • 單一細胞分離技術的CAPEX預算
  • 單一細胞解析技術的CAPEX預算
  • 單一細胞分離設備技術的市場估計
  • 單一細胞解析設備技術的市場估計
  • 單一細胞分離技術的消耗品預算
  • 單一細胞解析技術的消耗品預算
  • 單一細胞分離技術的消耗品的市場估計
  • 單一細胞解析技術的消耗品的市場估計
  • 單一細胞分離技術的主要供應商
  • 單一細胞解析技術的主要供應商
  • 為了學習單一細胞解析技術利用的資源
  • 為了收集單一細胞解析技術的資訊所參加的會議
  • 訂閱的雜誌·定期性點閱的網站
  • 對單一細胞技術追求的改良點和未滿足需求
  • 調查結果的摘要

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目錄

Executive Summary

This market report summarizes the results of HTStec's 2nd industry-wide global web-based benchmarking survey on single cell technologies carried out in September 2014.

The survey was initiated by HTStec as part of its tracking of emerging life science marketplaces and to update our earlier report (dated July 2012).

The objectives were to understand current interest and requirements for single cell technologies.

The questionnaire was compiled to meet the needs and interests of our clients, who were manufacturers/developers of laboratory equipment, reagents and consumables for the separation and analysis of single cells. They seek to gain insight in the application requirements, market opportunities, unmet needs and demand for single cell technology-related products.

Equal emphasis was given to soliciting opinion from all organizations interested in single cell separation or single cell analysis technologies with no geographical bias in the persons contacted.

The survey looked at the following aspects of single cell technologies, as practiced today (2014) and in a few cases as predicted for the future (2016): current use of single cells or single cell enabling technologies; market segments that best match respondents use and categorization of single technologies; scientific research area where interest in single cell technologies best fits; importance placed on the use/application of single cell technologies; main reasons for using a single cell versus an average of a pooled population of many cells; the value of the specific information derived by single cell technologies and whether it can be directly compared to pooled population of many cells; current use of technologies to separate single cells; analysis technologies applied to single cells; are available instrumentation for downstream (post-separation) single cell analysis fit for purpose; cell types used for single cell applications; main sources of isolated single cells; level of purity acceptable in isolated single cells; intended downstream work on isolated single cells; main applications of single cells and where single cells will impact most in the future; functions of a single cell enabling technology of greatest importance to respondent's research; number of single cells needed per experiment and frequency of running single cell experiments; destination substrate or vessel for isolated single cell delivery and transfer liquid volume per cell; main limitations of currently available single cell separation and analysis technologies; likelihood of purchasing any new instrumentation to enable single cell separation or single cell analysis; capex and consumable budgets for single cell separation or single cell analysis; awareness of key players (vendors) in the field of single cell separation or single cell analysis technologies; resources used to learn about new single cell technologies; conferences attended in the past 12 months in the pursuit of single cell technology information gathering/debate; publications respondents subscribe to, regularly read or visit online; and any improvements respondents require or unmet needs in single cell technologies.

The main questionnaire consisted of 29 multi-choice questions and 1 open-ended question. In addition, there were 7 questions related solely to survey demographics.

The survey collected 147 validated responses, of these 76% provided comprehensive input.

Survey responses were geographically split: 43% Europe; 41% North America; 10% Asia (excluding Japan & China); 3% Japan; 3% Rest of World; and 1% China.

Survey respondents were drawn from persons or groups currently using or planning future use of single cells or single cell enabling technologies in their research efforts.

Respondents came from 90 University/Research Institute/Not-for-Profit Facilities; 20 Biotechs; 12 Pharma; 7 Diagnostics; 6 Hospitals/Clinics; 4 Government/Military/Defence Facilities; 3 Contract Research Organisations; 3 Other; 1 Chemicals; and 1 Agri Biotech/Plant Genomics.

Most survey respondents had a senior job role or position which was in descending order: 27 professors/ assistant professors; 23 research scientists; 18 principal investigators; 16 senior scientists/research associates; 16 other roles; 13 post-docs; 10 directors; 9 section/group leaders; 6 department heads; 4 graduate students; 2 lab/research managers; and 2 vice presidents.

Survey results were expressed as an average of all survey respondents. In addition, where appropriate the data was fully reanalyzed after sub-division into 6 survey groups: 1) Basic Research; 2) Applied Research; 3) Pharma Research; 4) Clinical Research; 5) Primarily Genomic; & 6) Primarily Proteomic.

64% of respondents were currently using single cells or single cell technologies in their research.

Respondents were segmented on the basis of their current field of interest and use of single cell technologies: 44% basic research, 33% applied research; 12% clinical research; & 10% pharma research.

Respondent's interest can be further categorized as 36% primarily genomic and 13% primarily proteomic.

The scientific area where respondent's interest in single cell technologies most fits was oncology/cancer.

Most respondents placed high importance on the use of single cell technologies today.

Reveals intracellular differences in a heterogeneous population was ranked the main reason for using a single cell versus an average of a pooled population of many cells.

Most respondents rate the value of specific information derived from single cell technologies highly versus the average of a pooled population of many cells.

Most respondents thought information derived by single cell technologies can be compared to an average of a pooled population of many cells, but it is unlikely they will show >50% complementary.

FACS/flow cytometry was rated as the technology currently most used to isolate single cells from tissues or liquid culture today.

The analysis technologies most applied to single cells were fluorescence microscopy, FACS/flow cytometry and brightfield microscopy.

Of available instrumentation for single cell analysis fluorescence microscopy was rated as most adequate.

The most used cell type for single cell technology applications was primary cells.

The source most used to isolate single cells from was adherent cell cultures.

The median levels of acceptable purity in isolated single cells were as follows: desired cell phenotype >99.5%; cells that are singlet (not clumped) >99.5%; cells free from attached debris >99%; viable cells >95%; and cell cycle stage-specific >95%.

Most downstream work on isolated cells involves their immediate destruction to extract/purify cellular components for analysis.

Gene expression profiling was the application most applied/investigated with single cell technologies.

Biomarker discovery, validation and/or screening were the application expected to be impacted most by single cell technologies over the coming years.

The ability to pick a single cell with a specific phenotype from a heterogeneous cell mixture was rated as the function of a single cell enabling technology of greatest importance to respondent's research.

The median current or potential use of single cells were as follows: 100 isolated single cells per experiment; approximately 1 single cell experiment per week; main destination substrate/vessel for isolated single cells - 96-well plate; and transfer liquid volume per cell - 100nL-1uL.

High cost of equipment was rated the most limiting aspect of single cell separation technologies.

High cost per experiment was rated the most limiting aspect of single cell analysis technologies.

The likelihood respondents will purchase any new instruments for single cell separation or single cell analysis over the next few years (up to 2016) was possible (26-50% probability).

The median actual 2013 annual capex (instrument) budgets were $10K-$25K for single cell separation technologies and $10K-$25K for single cell analysis technologies.

The median actual annual consumable budgets spent in 2013 were $5K-$10K for single cell separation technologies and $5K-$10K for single cell analysis technologies.

Several bottom-up models were developed around the respondent's budgets to calculate the global single cell technologies markets. In 2014 these were estimated for single cell separation to be around $126M for instruments and $78M for consumables. In 2014 these were estimated for single cell analysis to be around $98M for instruments and $83M for consumables. Segmentation & CAGR estimates are given in the full report.

The top 3 vendors for single cell separation were Becton Dickenson, Fluidigm and Miltenyi Biotec.

The top 3 vendors for single cell analysis were Fluidigm, Life Technologies and Becton Dickenson.

Peer-reviewed publications were the resource most used to learn about new single cell technologies.

Most respondents have not attended any conferences in the last 12 months in the pursuit of new single cell technology information/debate.

The publications respondents subscribe to, regularly read or visit online most were Nature and Science.

Feedback on improvements required and unmet needs in single cell technologies were documented. One theme to emerge was the lack of a clear vision on the potential impact/value/applicability of single cell research.

The full report provides the data, details of the breakdown of the responses for each question, its segmentation and some estimates for the future (2016). It also highlights some interesting differences between the survey groups.

Table of Contents

  • Executive Summary
  • Table of Contents
  • Survey Methodology
  • Respondent's Organisational Origin And Survey Response
  • Respondent's Geographic Origin
  • Respondent's Company Or Organisational Origin
  • Respondent's Job Role
  • Respondents Currently Using Single Cells Or Single Cell Technologies
  • Current Field Of Interest & Use Of Single Cell Technologies
  • Respondent's Single Cell Research Application Categorization
  • Research Area Where Interest In Single Cell Technologies Best Fits
  • Importance Placed On The Use Or Application Of Single Cell Technologies
  • Main Reason For Using A Single Cell vs. An Averaged Pooled Population
  • Value Of Specific Information Derived From Single Cell Technologies
  • Can Information Derived By Single Cell Technologies Be Compared To Alternative Approaches
  • Technologies Currently Used To Isolate Single Cells From Tissues Or Liquid Culture Today
  • Analysis Technologies Applied To Single Cells (1)
  • Analysis Technologies Applied To Single Cells (2)
  • Single Cell Analysis Technologies That Are Fit For Purpose
  • Summary Of Survey Findings (1)
  • Cell Types Used For Single Cell Technology Applications
  • Sources Used To Isolate Single Cells
  • Level Of Purity Acceptable In Single Isolated Cells
  • What Downstream Work On Isolated Cells Involves
  • Applications Applied To Single Cells (1)
  • Applications Applied To Single Cells (2)
  • Applications Where Single Cell Technologies Are Expected To Impact (1)
  • Applications Where Single Cell Technologies Are Expected To Impact (2)
  • Functions Of A Single Cell Technology Of Greatest Importance
  • Current Or Potential Use Of Single Cells (1)
  • Current Or Potential Use Of Single Cells (2)
  • Current Or Potential Use Of Single Cells (3)
  • Current Or Potential Use Of Single Cells (4)
  • Main Limitations Of Currently Available Single Cell Separation Technologies
  • Main Limitations of Currently Available Single Cell Analysis Technologies
  • Summary of Survey Findings (2)
  • Likelihood Of Purchasing Single Cell Instruments
  • Capex (Instrument) Budget For Single Cell Separation Technologies
  • Capex (Instrument) Budget For Single Cell Analysis Technologies
  • Market Estimate For Single Cell Separation Instrument Technologies
  • Market Estimate For Single Cell Analysis Instrument Technologies
  • Consumables Budget For Single Cell Separation Technologies
  • Consumables Budget For Single Cell Analysis Technologies
  • Market Estimate For Single Cell Separation Consumables
  • Market Estimate For Single Cell Analysis Consumables
  • Top Vendors For Single Cell Separation Technologies (1)
  • Top Vendors For Single Cell Separation Technologies (2)
  • Top Vendors For Single Cell Analysis Technologies (1)
  • Top Vendors For Single Cell Analysis Technologies (2)
  • Resources Used To Learn About New Single Cell Technologies
  • Conferences Attended To Gather Information On Single Cell Technologies
  • Publications Respondents Subscribe To, Regularly Read Or Visit Online
  • Improvements Wanted & Unmet Needs In Single Cell Technologies
  • Summary of Survey Findings (3)
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