高容許量蛋白質精製

支援技術

1月11日∼12日

要實現高容許量蛋白質處理，使各階段效率化、確保品質、短期能獲得需要的成果，需要各種手法及支援技術。該部會檢討在高容許量(HTP)中使精製工程最佳化時可利用的策略，同時監視蛋白質的處理工程，分析結果，以及表示蛋白質狀態和生物活性的各種手法。此外，包含各種軟體和LIMS（實驗室資訊管理系統）數據的繼續收集和處理技術，再加上選擇複製品（clone）和緩衝的最適化、標籤的刪除等見識，亦採用液體處理系統和微流體技術等容許量自動化、引進單回使用技術和一次性技術等主題。

各種見識和技術創新

OPENING KEYNOTE PRESENTATION: High-Throughput Screening for Chromatographic and Non-Chromatographic Protein Purification Methods

Steven Cramer, Ph.D., William Weightman Walker Professor, Chemical and Biological Engineering, Rensselaer Polytechnic Institute

FEATURED PRESENTATION: High-Throughput Cloning, Expression and Purification: An Industry Perspective

Andrew Fosberry, Ph.D., Manager, Expression and Fermentation Sciences, GlaxoSmithKline

Beyond the Plan, What Really Happens in an HTP Project: Case Studies from a Core Service Lab

William Gillette, Ph.D., Senior Scientist, Protein Expression Lab, SAIC-Frederick, Inc.

難處理的蛋白質高容許量之精製

High-Throughput Expression and Purification of Membrane Proteins

Filippo Mancia, Ph.D., Assistant Professor, Physiology & Cellular Biophysics, Columbia University

支援技術

Test Method Development for High-Throughput Processes

Steven S. Kuwahara, Ph.D., Principal, QC, GXP Biotechnology LLC

Protein Arrays for Assessment of Human Anti-Viral Antibody Responses

Jonathan S. Duke-Cohan, Ph.D., Department of Medical Oncology, Dana-Farber Cancer Institute, Harvard Institutes of Medicine

高容許量製程的開發

Identification of Chromatographic Steps for Protein Purification using Parallel Screening Methods

Kaushal Rege, Ph.D., Assistant Professor, Chemical Engineering, Ira A. Fulton School of Engineering, Arizona State University

IT Partnership to Enable High-Throughput Process Development

Paul Hanson, Ph.D., Staff Engineer II, Cambridge Biologics CMC Group, Millennium Pharmaceuticals

高容許量製程的自動化

Automating Protein Purification for Genomic Enzymology Research

Sirano Dhe-Paganon, Ph.D., Assistant Professor, Physiology, University of Toronto