基因、傳染媒介（vector）、構造體，複製品（clone）的開發

與下游成功有關之上游的判斷

1月9日∼10日

對生物學療法可利用之高品質蛋白質的需求已擴大到未曾有過的規模。機能性蛋白質的生產如要達成提升到比迄今更高水準的目標，需要工學(科學)和洞察(方法論)。生物療法用蛋白質有效的發現之課題，是與科學及方法論都有關的主題。此部會沿襲上次生物療法用機能性蛋白質產品之蛋白質發現研究時應用蛋白質工學有效的策略之主題，能學習到由經驗豐富的研究人員發表現場的經驗及應用技術、研究成果等。

基因的標的化

Efficient Optimization of Protein Expression Using High-Throughput Gene Synthesis and Screens

Jingdong Tian, Ph.D., Assistant Professor, Biomedical Engineering, Duke University

Semi-Automatic Cloning and Expression of Immunoglobulin Genes from Single-Plasma Cells for High-Throughput Generation of Monoclonal Antibodies

Masaharu Isobe, Ph.D., Professor, Molecular and Cellular Biology, Life Sciences and Bioengineering, University of Toyama

傳染媒介（vector）的構成

The Use of a Combinatorial Recombinational Cloning Platform to Simplify Construction of Expression Vectors for Wide-Ranging Areas of Proteomic and Genetic Research

Dominic Esposito, Ph.D., Interim Director, Protein Expression Laboratory; Group Leader, Clone Optimization Group, Advanced Technology Program, SAIC-Frederick, Inc.

Parallel Construct Expression Evaluation Using a High-Throughput Process for Baculovirus-Infected Insect Cells

W. Clay Brown, Ph.D., High-Throughput Protein Lab, Center for Structural Biology, Life Sciences Institute, University
of Michigan

Re-Engineering Multicloning Sites for Function and Convenience

Hal Alper, Ph.D., Assistant Professor, Chemical Engineering, University of Texas at Austin

So Many Options, So Little Money: How to Choose the Right Vector and Screen for Functional Proteins

Bill Gillette, Ph.D., Senior Scientist, Group Leader, Protein Expression Laboratory, Advanced Technology Program, SAIC-Frederick, Inc.

開發transient expression system

Maximizing Recombinant Protein Expression Through Systematic Gene Design

Mark Welch, Director, Gene Design, DNA2.0

Engineering and Evaluation of Mammalian Secreted Protein Expression Library for High-Throughput Target Discovery

Tadas Panavas, Ph.D., Senior Scientist, Molecular and Protein Biosciences, Centocor R&D, Inc.

Development of Expression-Ready Constructs for Generation of Proteomic Libraries

Susan Celniker, Ph.D., Co-Director, Life Sciences, Lawrence Berkeley National Laboratory

Driving a Variant of the Methanol-Inducible AOX1 Promoter from Pichia pastoris with Glycerol for High-Level Expression of (Therapeutic) Proteins

Roland Weis, Ph.D., Head, Operations, VTU Technology GmbH, Austria

Influence of Histidine Affinity Tags on Protein Structural Order

Evelina Angov, M.S., Ph.D., Microbiologist, Malaria Vaccine Development, Walter Reed Army Institute of Research