宿主和平台的選擇、設計、最適化

1月11日∼12日

蛋白質的研究人員正面臨要在更短期能大量生產蛋白質的課題。依情況有時需要新的技術和策略，然藉由使現有的供給源再生或再設計，亦有可能增加生產量。最重要的是選擇發現蛋白質的宿主時要作出聰明且有效的判斷。此部會檢討可能是宿主/系統最佳選擇的最新數據和創新的技術，同時考量如何「挽救」現有系統，更有效改善機能，以及提高生產蛋白質的質和量之方法。

原核生物成果的最大化

Engineering of Escherichia coli for Endotoxin-Free Production of Recombinant Proteins and Plasmid DNA

Uwe Mamat, Ph.D., Research Professor, Center for Medicine and Biosciences, Research Center Borstel

Transient Expression of an E. coli Periplasmic Expression of Fab�f Fragments at 2-5g/l

Mark Ellis, Senior Scientist, Protein Expression and Purification, UCB Celltech

Discovery Protein Expression Enabled by Pfenex Expression Technology™

Diane Retallack, Ph.D., Director, Molecular Biology, Pfenex, Inc.

擴大對真核生物的投資收益

Early Markers of Production Instability of Recombinant Chinese Hamster Ovary Cell Line

Ulrich Göpfert, Ph.D., Pharma Research and Early Development, Roche Diagnostics GmbH

Optimizing the Baculovirus-Insect Cell Platform for Recombinant Glycoprotein Expression

Donald Jarvis, Ph.D., Professor, Molecular Biology, University of Wyoming

The Baculovirus Expression Platform: Recent Advances & More Lessons Learned

James M. Groarke, Ph.D., Biortus Biosciences

Generation and Screening of Pichia pastoris Strains with Enhanced Protein Production by Use of Microengraving

J. Christopher Love, Ph.D., Associate Professor, Department of Chemical Engineering, Massachusetts Institute of Technology

加強發現高容許量

Maximizing the Success of Protein Expression in High-Throughput Fashion

Yvonne Franke, Ph.D., Scientist Technology, Structural Biology, Genentech, Inc.

Improving Protein Production in Insect and Mammalian Cells using Combinatorial Libraries of Expression-Enhancing Elements

Dominic Esposito, Ph.D., Director, Protein Expression Laboratory, SAIC-Frederick, Inc.

Self-Cleaning Fermentors and Auto-Inducing Media for High-Throughput Parallel Expression in 1–5 l Scale

Alvar Gossert, Ph.D., Investigator, Structural Biology Platform, Novartis Institutes for BioMedical Research