克服課題與探求解決方案

1月12日∼13日

有必要治療和診斷的大半蛋白質，很難在原有的宿主身上發現，存在摺疊（folding）、溶解性和無法進行醣化作用（Glycosylation）等問題，而且對宿主都算是有毒性的東西。此部會介紹蛋白質科學領域的最新成果，發表克服這些課題的見解與解決方案。

重點發表

Protein Expression in Drug Discovery – New Challenges, New Solutions

Lorenz Mayr, Ph.D., Executive Director, CPC/EPP, Novartis Pharma AG

Enhanced Glycoprotein Production in HEK-293 Cells Expressing Pyruvate Carboxylase

Yves Durocher, Ph.D., Chemical Engineering, École Polytechnique de Montréal

因應溶解性、摺疊（folding）、無法進行醣化作用（Glycosylation）問題的解決方案

Expression of Soluble Proteins by Linkage with Acidic Tags

Richard Discipio, Ph.D., Research Scientist, Torrey Pines Institute for Molecular Studies

Measurement of Protein Unfolding/Refolding Kinetics and Structural Characterization of Hidden Intermediates by NMR Relaxation Dispersion

Derrick Meinhold, Ph.D., Research Associate, Department of Molecular Biology and Skaggs Institute of Chemical Biology, The Scripps Research Institute

Effects of Cell Culture Conditions on Antibody N-Linked Glycosylation—What Affects High Mannose 5 Glycoform?

Efren Pacis, Biologics Pharma Technical Development, Genentech, Inc.

解決膜蛋白質的問題

Restrained Expression: A Method to Overproduce Toxic Membrane Proteins by Exploiting Operator-Repressor Interactions

Dinesh A. Yernool, Ph.D., Assistant Professor, Department of Biological Sciences, Purdue University

Reprogramming Chaperone Pathways to Improve Membrane Protein Expression in Escherichia coli

Francois Baneyx, Ph.D., Professor, Chemical Engineering, University of Washington, Seattle

因應蛋白質凝集特性描述（characterization）之分析法的課題和進步

Aggregates and Their Analysis: Some Perspectives and Lessons from Working with 100+ Products

John Philo, Ph.D., Vice President, Director of Biophysical Chemistry, Alliance Protein Laboratories

Addressing New Analytical Challenges in Protein Formulation Development

Speaker to be Announced

Characterization of Submicron Particle Distributions in Biologics Formulations

Flaviu Gruia, Ph.D., Scientist, Analytical Biochemistry, MedImmune

Characterization of Sub-Visible Particles in Therapeutic Protein Formulations

Tobias Frommknecht, Ph.D., Formulation Scientist, pRED, Hoffmann-La Roche Ltd